The need for an accurate, fast and reliable analysis of carbohydrate test is crucial for
numerous biological processes. In that sense, anthrone–sulfuric acid assay is one of the …

A murine monoclonal antibody developed for the purification of recombinant hepatitis B
surface antigen was immobilized on a chromatographic support and used to adsorb and …

A comparison between expanded bed adsorption and conventional packed bed Protein A
Fast Flow to purify the anti-rHBsAg mAbs from feedstock is presented in this work. Direct …

An immunoaffinity chromatographic method was developed using a mAb immunosorbent to
purify recombinant hepatitis B surface antigen (r-HBsAg) from yeast. Elution conditions using …

The virus removal of protein A affinity chromatography, inactivation capacity, acid pH and a
combination of high temperature with a chaotropic agent was determined in this work. The …

An enzyme-linked immunosorbent assay to quantify the mAb CB. Hep-1 during downstream
purification process was standardized and validated. This assay is characterized by a short …

Recombinant hepatitis B surface antigen (r‐HBsAg) produced in yeast is adsorbed on a
diatomaceous earth matrix for purification purposes. A pH dependence in the adsorption …

The protein-free medium TurboDoma HP. 1 (THP. 1) was used to produce the CB. Hep-1
monoclonal antibody (mAb) in a CP-1000 hollow fiber bioreactor (HFB). This mAb is used for …

The conformational stability of a single-chain Fv antibody fragment against a hepatitis B
surface antigen (anti-HBsAg scFv) has been studied by urea and temperature denaturation …

In this paper we describe the antigen recognition characteristics, variable region base and
amino acid sequence, and performance as immunoaffinity chromatography ligands of two …