Methods of endotoxin detection

W Su, X Ding - Journal of laboratory automation, 2015 - journals.sagepub.com
Endotoxin, present in the outer membrane of all gram-negative bacteria, can pose serious
risks to human health, from irreversible shock to death. Therefore, it is essential to develop …

Current trends in endotoxin detection and analysis of endotoxin–protein interactions

EC Dullah, CM Ongkudon - Critical reviews in biotechnology, 2017 - Taylor & Francis
Endotoxin is a type of pyrogen that can be found in Gram-negative bacteria. Endotoxin can
form a stable interaction with other biomolecules thus making its removal difficult especially …

Electrochemical biosensor for trypsin activity assay based on cleavage of immobilized tyrosine-containing peptide

TA Filippova, RA Masamrekh, VV Shumyantseva… - Talanta, 2023 - Elsevier
In this work, we proposed a biosensor for trypsin proteolytic activity assay using
immobilization of model peptides on screen-printed electrodes (SPE) modified with gold …

Selective sensing of copper ions by mesoporous porphyrinic metal–organic framework nanoovals

Z Xu, QY Meng, Q Cao, Y Xiao, H Liu, G Han… - Analytical …, 2019 - ACS Publications
Copper is a common element in the environment and human body. Exposure to high
concentrations of Cu2+ potentially causes health issues, such as Wilson and Alzheimer's …

Electrochemical approach for the development of a simple method for detecting cell apoptosis based on caspase-3 activity

S Takano, S Shiomoto, KY Inoue, K Ino… - Analytical …, 2014 - ACS Publications
This paper reports a novel approach for the simple detection of cell apoptosis using an
electrochemical technique. This method uses caspase-3 activity as an indicator of apoptosis …

An aptamer based voltammetric biosensor for endotoxins using a functionalized graphene and molybdenum disulfide composite as a new nanocarrier

Y Yuan, L Li, M Zhao, J Zhou, Z Chen, L Bai - Analyst, 2019 - pubs.rsc.org
Lipopolysaccharides (LPS), known as endotoxins, can cause a strong inflammatory
response and lead to multiple organ failure in severe cases. This work reports a simple label …

A colorimetric strategy for assay of protease activity based on gold nanoparticle growth controlled by ascorbic acid and Cu (II)-coordinated peptide

L Liu, D Deng, Y Wang, K Song, Z Shang… - Sensors and Actuators B …, 2018 - Elsevier
We developed a colorimetric method for assay of protease activity through the growth of gold
nanoparticles (AuNPs) with ascorbic acid (AA) as the reducing agent. The method is based …

Changing global perspectives on horseshoe crab biology, conservation and management

RH Carmichael, ML Botton, PKS Shin, SG Cheung - 2015 - Springer
Since the first International Symposium on Science and Conservation of Horseshoe Crabs in
New York, USA, in 2007, we have driven and witnessed a revolution in horseshoe crab …

Modeling of an electrochemical nanobiosensor in COMSOL Multiphysics to determine phenol in the presence of horseradish peroxidase enzyme

A Kaffash, K Rostami, HR Zare - Enzyme and microbial technology, 2019 - Elsevier
Horseradish peroxidase enzyme selectively oxidizes phenol to o-quinone that can be
reduced electrochemically to catechol and generating a current response which is directly …

A facile method for fabricating carbon fiber-based gold ultramicroelectrodes with different shapes using flame etching and electrochemical deposition

VK Aydin, M Şen - Journal of Electroanalytical Chemistry, 2017 - Elsevier
In this study, we developed a simple, cost-efficient, and time-saving approach for producing
gold (Au) ultramicroelectrodes (UMEs) with different shapes and sizes ranging from one …