BACKGROUND Cellular life depends on the ability of organisms to accurately duplicate and
transmit genetic information between generations. In bacteria, archaea, and eukaryotes, this …

New antibiotics with novel modes of action are required to combat the growing threat posed
by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high …

Dedicated AAA+ ATPases deposit hexameric ring-shaped helicases onto DNA to promote
replication in cellular organisms. To understand how loading occurs, we used electron …

In cells, dedicated AAA+ ATPases deposit hexameric, ring-shaped helicases onto DNA to
initiate chromosomal replication. To better understand the mechanisms by which helicase …

Threading of DNA through the central channel of a replicative ring helicase is known as
helicase loading, and is a pivotal event during replication initiation at replication origins …

Initiation of DNA replication is required for cell viability and passage of genetic information to
the next generation. Studies in Escherichia coli and Bacillus subtilis have established A …

During the assembly of the bacterial loader-dependent primosome, helicase loader proteins
bind to the hexameric helicase ring, deliver it onto the ori C DNA and then dissociate from …

Helicase loading at a DNA replication origin often requires the dynamic interactions
between the DNA helicase and an accessory protein. In E. coli, the DNA helicase is DnaB …

Hexameric helicases control both the initiation and the elongation phase of DNA replication.
The toroidal structure of these enzymes provides an inherent challenge in the opening and …

Highlights•Hexameric nucleic-acid translocases and helicases form rings that encircle DNA
or RNA.•These motors hydrolyze ATP to power nucleic acid movement through a central …