Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Numerous strategies for linking desired chemical probes with target peptides and proteins
have been developed and applied in the field of biological chemistry. Approaches for site …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

The cell wall is a critical extracellular barrier for bacteria and many other organisms. In
bacteria, this structural layer consists of peptidoglycan, which maintains cell shape and …

Conferring multifunctional properties to proteins via enzymatic approaches has greatly
facilitated recent progress in protein nanotechnology. In this regard, sortase (Srt) A …

Enzymatic site-specific bioconjugation techniques, in particular sortase-mediated ligation,
are increasingly used to generate conjugated proteins for a wide array of applications …

Gram-positive bacteria contain sortase enzymes on their cell surfaces that catalyze
transpeptidation reactions critical for proper cellular function. In vitro, sortases are used in …

Protein biomaterials would have the potential to address global challenges in health and
environment. Numerous production methods of the biomaterials exist, with sortase-mediated …

Gram-positive bacteria are some of the earliest known life forms, diverging from gram-
negative bacteria 2 billion years ago. These organisms utilize sortase enzymes to attach …

Quantitative and selective labelling of proteins is widely used in both academic and
industrial laboratories, and catalytic labelling of proteins using transpeptidases, such as …