Engineered living biomaterials
A Rodrigo-Navarro, S Sankaran, MJ Dalby… - Nature Reviews …, 2021 - nature.com
Biomaterials have evolved from inert materials that lack interaction with the body to
biologically active, instructive materials that host and provide signals to surrounding cells …
biologically active, instructive materials that host and provide signals to surrounding cells …
Reconstructing organisms in silico: genome-scale models and their emerging applications
X Fang, CJ Lloyd, BO Palsson - Nature Reviews Microbiology, 2020 - nature.com
Escherichia coli is considered to be the best-known microorganism given the large number
of published studies detailing its genes, its genome and the biochemical functions of its …
of published studies detailing its genes, its genome and the biochemical functions of its …
A comprehensive genome‐scale reconstruction of Escherichia coli metabolism—2011
The initial genome‐scale reconstruction of the metabolic network of Escherichia coli K‐12
MG1655 was assembled in 2000. It has been updated and periodically released since then …
MG1655 was assembled in 2000. It has been updated and periodically released since then …
[HTML][HTML] Type VI Secretion System in Pathogenic Escherichia coli: Structure, Role in Virulence, and Acquisition
F Navarro-Garcia, F Ruiz-Perez, Á Cataldi… - Frontiers in …, 2019 - frontiersin.org
Bacterial pathogens utilize a myriad of mechanisms to invade mammalian hosts, damage
tissue sites, and evade the immune system. One essential strategy of Gram-negative …
tissue sites, and evade the immune system. One essential strategy of Gram-negative …
Genome-scale metabolic reconstructions of multiple Escherichia coli strains highlight strain-specific adaptations to nutritional environments
Genome-scale models (GEMs) of metabolism were constructed for 55 fully sequenced
Escherichia coli and Shigella strains. The GEMs enable a systems approach to …
Escherichia coli and Shigella strains. The GEMs enable a systems approach to …
A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli
Q Li, B Sun, J Chen, Y Zhang… - Acta Biochimica et …, 2021 - academic.oup.com
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …
[HTML][HTML] Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter …
Background Predictable control of gene expression is necessary for the rational design and
optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of …
optimization of cell factories. In the yeast Saccharomyces cerevisiae, the promoter is one of …
[HTML][HTML] Multi-omics quantification of species variation of Escherichia coli links molecular features with strain phenotypes
Escherichia coli strains are widely used in academic research and biotechnology. New
technologies for quantifying strain-specific differences and their underlying contributing …
technologies for quantifying strain-specific differences and their underlying contributing …
[HTML][HTML] Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …
[HTML][HTML] Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction
Background Microbial consortia composed of autotrophic and heterotrophic species abound
in nature, yet examples of synthetic communities with mixed metabolism are limited in the …
in nature, yet examples of synthetic communities with mixed metabolism are limited in the …