Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Understanding the function of biological macromolecules and their complexes at the
physicochemical level requires knowledge of both their structure and dynamics …

Paramagnetic centers in biomolecules, such as specific metal ions that are bound to a
protein, affect the nuclei in their surrounding in various ways. One of these effects is the …

Myriad biological processes proceed through states that defy characterization by
conventional atomic-resolution structural biological methods. The invisibility of these …

NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …

Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Page 1 1
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags Christoph …

The advent of different lanthanide-binding reagents has made site-specific labelling of
proteins with paramagnetic lanthanides a viable proposition. This brings many powerful …

We investigate complexes of two paramagnetic metal ions Gd3+ and Mn2+ to serve as
polarizing agents for solid-state dynamic nuclear polarization (DNP) of 1H, 13C, and 15N at …

Immobilized lanthanide ions offer the opportunity to refine structures of proteins and the
complexes they form by using restraints obtained from paramagnetic NMR experiments. We …

Paramagnetic effects offer a rich source of long-range structural restraints. Here we review
current methods for site-specific tagging of proteins and oligonucleotides with paramagnetic …