The DNA sequences associated with 139 apparent streptomycete transcriptional start sites
are compiled and compared. Of these, 29 promoters appeared to belong to a group which …

The main features that characterize rolling‐circle replication (RCR)(see Fig. 1A) derive from
its singular initiation mechanism, which relies on the sequence‐specific cleavage, at the nick …

SEQUENCE ALIGNMENT The multiple alignments ofthe amino acid sequences for this
update were performed in most cases by merely adding on to our original alignments (1) …

Summary The 3'~ 5'exonuclease active site of E. coli DNA polymerase I is predicted to be
conserved for both prokaryotic and eukaryotic DNA polymerases based on amino acid …

E. coli DNA polymerase IH is a multisubunit enzyme encoded by various dna genes (55); the
DNA polymerizing a-subunit encoded by thepolC (dnaE) gene (56) and the 3' …

The Klenow fragment structure, together with many biochemical experiments, has suggested
a region of the protein that may contain the polymerase active site. We have changed 7 …

In addition to the general 3′–5′ exonuclease domain described by Bernad et al.[Cell 59
(1989) 219–228] significant amino acid (aa) sequence similarity has been found in the C …

The genetic cassette encoding theDpnII restriction–modification system ofStreptococcus
pneumoniaegave transcription products of approximately 2.7 and 1.8 kilobases. The larger …

Deoxy-and dideoxynucleotides differ only in whether they have a hydroxyl substituent at C-
3′ of the ribose moiety, and yet the Klenow fragment DNA polymerase prefers the natural …

[57] ABSTRACT A means for cleaving a nucleic acid cleavage structure in a site-speci? c
manner is disclosed. A cleaving enzyme having 5'nuclease activity without interfering …