Pichia pastoris is extensively used to produce various heterologous proteins. Amounts of
biopharmaceutical drugs and industrial enzymes have been successfully produced by fed …

Recent noteworthy advances in developing high‐performing microbial and mammalian
strains have enabled the sustainable production of bio‐economically valuable substances …

This work proposes a hybrid observer based on an asymptotic observer combined with an
extended Kalman filter applied to high-density cultures of S. cerivisiae. The observer …

Bio‐based production of fuels, chemicals and materials is needed to replace current fossil
fuel based production. However, bio‐based production processes are very costly, so the …

Abstract The methylotrophic yeast Komagataella (Pichia) pastoris has become one of the
most utilized cell factories for the production of recombinant proteins over the last three …

The separation and purification of recombinant pharmaceutical proteins is a fundamental
and challenging step in the biotechnology industry. Hierarchical nanostructures with unique …

Background: Process development involving system metabolic engineering and bioprocess
engineering has become one of the major thrust for the development of therapeutic proteins …

Exact monitoring of the key process variables, such as specific growth rate (μ), is essential
for effective process control, and maintenance of the optimal conditions for the production of …

Genome‐scale metabolic models are valuable tools for the design of novel strains of
industrial microorganisms, such as Komagataella phaffii (syn. Pichia pastoris). However, as …

The cell culture medium is an intricate mixture of components which has a tremendous effect
on cell growth and recombinant protein production. Regular cell culture medium includes …