Validation of a species-specific probe-based qPCR assay for the threatened meltwater stonefly,...

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The unknown unknown: A framework for assessing environmental DNA assay specificity against unsampled taxa

TM Wilcox, JA Kronenberger, MK Young… - Molecular Ecology …, 2024 - Wiley Online Library
Taxon‐specific quantitative PCR (qPCR) assays are commonly used for environmental DNA
sampling‐based inference of animal presence. These assays require thorough validation to …
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