[HTML][HTML] Multiplexed microsphere-based flow cytometric assays

KL Kellar, MA Iannone - Experimental hematology, 2002 - Elsevier
Flow cytometry has become an indispensable tool for clinical diagnostics and basic
research. Although primarily designed for cellular analysis, flow cytometers can detect any …

The genesis and evolution of bead-based multiplexing

H Graham, DJ Chandler, SA Dunbar - Methods, 2019 - Elsevier
Multiplexed analysis has the advantage of allowing for simultaneous detection of multiple
analytes in a single reaction vessel which reduces time, labor, and cost as compared to …

Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells

W De Jager, H te Velthuis, BJ Prakken… - Clinical and Vaccine …, 2003 - Am Soc Microbiol
Cytokines secreted by cells of the immune system can alter the behavior and properties of
immune or other cells. At a site of inflammation, sets of cytokines interact with immune cells …

Mode of delivery modulates the intestinal microbiota and impacts the response to vaccination

EM de Koff, D van Baarle, MA van Houten… - Nature …, 2022 - nature.com
The gut microbiota in early life, when critical immune maturation takes place, may influence
the immunogenicity of childhood vaccinations. Here we assess the association between …

Validation and comparison of luminex multiplex cytokine analysis kits with ELISA: determinations of a panel of nine cytokines in clinical sample culture supernatants

K Wang, PD Wadhwa, JF Culhane, EL Nelson - Journal of reproductive …, 2005 - Elsevier
PROBLEM:: Analyses of the expression pattern of multiple cytokines are frequently required
for characterization of the status of the immune system as it pertains to Th type bias and …

Simultaneous quantitation of antibodies to neutralizing epitopes on virus-like particles for human papillomavirus types 6, 11, 16, and 18 by a multiplexed luminex …

D Opalka, CE Lachman, SA MacMullen… - Clinical and Vaccine …, 2003 - Am Soc Microbiol
Several different methods have been developed to quantitate neutralizing antibody
responses to human papillomaviruses (HPVs), including in vivo neutralization assays, in …

[图书][B] Advanced techniques in diagnostic microbiology

YW Tang, CW Stratton, YW Tang - 2013 - Springer
Medical microbiology is a branch of medical science that deals with the prevention,
diagnosis, and therapy of infectious diseases. A clinical microbiologist is a professional …

Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus

PGM van Gageldonk, FG van Schaijk… - Journal of …, 2008 - Elsevier
To increase testing of vaccine induced humoral immunity in immune surveillance studies
and vaccine trials, a rapid and simple microsphere-based multiplex assay (pentaplex) was …

Spectrum-resolved dual-color electrochemiluminescence immunoassay for simultaneous detection of two targets with nanocrystals as tags

G Zou, X Tan, X Long, Y He, W Miao - Analytical chemistry, 2017 - ACS Publications
A spectrum-resolved dual-color electrochemiluminescence (ECL) immunoassay was
designed and implemented to simultaneously detect carcinoembryonic antigen (CEA) and …

Development and validation of a nonaplex assay for the simultaneous quantitation of antibodies to nine Streptococcus pneumoniae serotypes

G Lal, P Balmer, E Stanford, S Martin… - Journal of …, 2005 - Elsevier
Serum IgG levels specific for Streptococcus pneumoniae are currently quantified using the
ELISA. However, this method has significant limitations in that a separate test is required for …