Navigating the landscape of enzyme design: from molecular simulations to machine learning

J Zhou, M Huang - Chemical Society Reviews, 2024 - pubs.rsc.org
Global environmental issues and sustainable development call for new technologies for fine
chemical synthesis and waste valorization. Biocatalysis has attracted great attention as the …

xTrimoPGLM: unified 100B-scale pre-trained transformer for deciphering the language of protein

B Chen, X Cheng, P Li, Y Geng, J Gong, S Li… - arXiv preprint arXiv …, 2024 - arxiv.org
Protein language models have shown remarkable success in learning biological information
from protein sequences. However, most existing models are limited by either autoencoding …

[HTML][HTML] Data-driven strategies for the computational design of enzyme thermal stability: Trends, perspectives, and prospects: Data-driven strategies for enzyme …

Z Dou, Y Sun, X Jiang, X Wu, Y Li, B Gong… - Acta Biochimica et …, 2023 - ncbi.nlm.nih.gov
Thermal stability is one of the most important properties of enzymes, which sustains life and
determines the potential for the industrial application of biocatalysts. Although traditional …

HybridGCN for protein solubility prediction with adaptive weighting of multiple features

L Chen, R Wu, F Zhou, H Zhang, JK Liu - Journal of Cheminformatics, 2023 - Springer
The solubility of proteins stands as a pivotal factor in the realm of pharmaceutical research
and production. Addressing the imperative to enhance production efficiency and curtail …

Enabling high-throughput enzyme discovery and engineering with a low-cost, robot-assisted pipeline

B Norton-Baker, MCR Denton, NP Murphy, B Fram… - Scientific Reports, 2024 - nature.com
As genomic databases expand and artificial intelligence tools advance, there is a growing
demand for efficient characterization of large numbers of proteins. To this end, here we …

Deep learning prediction of enzyme optimum pH

JE Gado, M Knotts, AY Shaw, D Marks, NP Gauthier… - bioRxiv, 2023 - biorxiv.org
The relationship between pH and enzyme catalytic activity, as well as the optimal pH (pHopt)
at which enzymes function, is crucial for biotechnological applications. Consequently …

REME: an integrated platform for reaction enzyme mining and evaluation

Z Shi, D Wang, Y Li, R Deng, J Lin, C Liu… - Nucleic Acids …, 2024 - academic.oup.com
A key challenge in pathway design is finding proper enzymes that can be engineered to
catalyze a non-natural reaction. Although existing tools can identify potential enzymes …

[HTML][HTML] DeepTM: A deep learning algorithm for prediction of melting temperature of thermophilic proteins directly from sequences

M Li, H Wang, Z Yang, L Zhang, Y Zhu - Computational and Structural …, 2023 - Elsevier
Thermally stable proteins find extensive applications in industrial production,
pharmaceutical development, and serve as a highly evolved starting point in protein …

A general temperature-guided language model to engineer enhanced stability and activity in proteins

P Tan, M Li, Y Yu, F Jiang, L Zheng, B Wu… - arXiv preprint arXiv …, 2023 - arxiv.org
Designing protein mutants with high stability and activity is a critical yet challenging task in
protein engineering. Here, we introduce PRIME, an innovative deep learning approach for …

Recent Advances in the Integration of Protein Mechanics and Machine Learning

YL Chen, SW Chang - Extreme Mechanics Letters, 2024 - Elsevier
Mechanics underlies protein properties and behavior. From a theoretical standpoint, it is
possible to derive these based on physical rules. This is appealing because they provide …