Developing greener synthesis processes is an inescapable necessity to transform the
industrial landscape, mainly in the pharmaceutical sector, into a long-term, sustainable …

Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

Proteins possess unique molecular recognition capabilities and enzymatic activities,
features that are usually tied to a particular tertiary structure. To make use of proteins for …

Enzymes are of central importance to many biotechnological and biomedical applications.
However, for many potential applications, the required conditions impede enzyme folding …

Designed ankyrin repeat proteins (DARPins) are genetically engineered proteins that exhibit
high specificity and affinity toward specific targets. Here, the G3-DARPin, which binds the …

Sortase-mediated ligation (sortagging) is commonly performed using the Staphylococcus
aureus sortase A (SaSrtA) that strictly recognizes the N-terminal glycine residue. In this work …

Engineering precise substrate specificity of proteases advances the potential to use them in
biotechnological and therapeutic applications. Collagen degradation, a physiological …

Gram‐positive bacteria experience considerable mechanical perturbation when adhering to
host surfaces during colonization and infection. They have evolved various adhesion …

Chemical synthesis of complex peptides and proteins continues to play increasingly
important roles in industry and academia, where strategies for covalent ligation of two or …