MS-based proteome profiling has become increasingly comprehensive and quantitative, yet
a persistent shortcoming has been the relatively large samples required to achieve an in …

Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Progress in proteomics is mainly driven by advances in mass spectrometric (MS)
technologies. Here we benchmarked the performance of the latest MS instrument in the …

Single-cell proteomics can provide unique insights into biological processes by resolving
heterogeneity that is obscured by bulk measurements. Gains in the overall sensitivity and …

To estimate absolute protein contents in complex mixtures, we previously defined a protein
abundance index (PAI) as the number of observed peptides divided by the number of …

Proteomic analysis of complex protein mixtures using proteolytic digestion and liquid
chromatography in combination with tandem mass spectrometry is a standard approach in …

The absolute need to improve the separating power of liquid chromatography, especially for
multi-constituent biological samples, is becoming increasingly evident. In response, over the …

Peak capacity is the best measure of the performance of a gradient separation. In this paper,
the theory of peak capacity for the standard operating conditions of reversed-phase and ion …

Selected reaction monitoring (SRM)–also known as multiple reaction monitoring (MRM)–has
emerged as a promising high‐throughput targeted protein quantification technology for …

▪ Abstract Mass spectrometry provides key tools for the analysis of proteins. New types of
mass spectrometers that provide enhanced capability to discover protein identities and …