The spliceosome removes introns from messenger RNA precursors (pre-mRNA). Decades
of biochemistry and genetics combined with recent structural studies of the spliceosome …

Recent improvements in experimental and computational techniques that are used to study
the transcriptome have enabled an unprecedented view of RNA processing, revealing many …

Motivation Protein-to-genome alignment is critical to annotating genes in non-model
organisms. While there are a few tools for this purpose, all of them were developed over 10 …

Understanding genome organization and gene regulation requires insight into RNA
transcription, processing and modification. We adapted nanopore direct RNA sequencing to …

To identify novel genes associated with ALS, we undertook two lines of investigation. We
carried out a genome-wide association study comparing 20,806 ALS cases and 59,804 …

Synonymous mutations change the sequence of a gene without directly altering the
sequence of the encoded protein. Here, we present evidence that these" silent" mutations …

Alternative splicing (AS) is a key regulatory mechanism that contributes to transcriptome and
proteome diversity. As very few genome-wide studies analyzing AS in plants are available …

Abstract We describe Trans-ABySS, a de novo short-read transcriptome assembly and
analysis pipeline that addresses variation in local read densities by assembling read …

Most eukaryotes harbor two distinct pre-mRNA splicing machineries: the major spliceosome,
which removes> 99% of introns, and the minor spliceosome, which removes rare …

Alternative splicing generates multiple transcript and protein isoforms from the same gene
and thus is important in gene expression regulation. To date, RNA-sequencing (RNA-seq) is …