The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Protein molecules often interact with other partner protein molecules in order to execute their
vital functions in living organisms. Characterization of protein–protein interactions thus plays …

Protein function is often regulated by posttranslational modifications (PTMs), and recent
advances in mass spectrometry have resulted in an exponential increase in PTM …

DNA deaminase enzymes play key roles in immunity and have recently been harnessed for
their biotechnological applications. In base editors (BEs), the combination of DNA …

Inspired by the remarkable ability of natural protein switches to sense and respond to a wide
range of environmental queues, here we report a strategy to engineer synthetic protein …

We report a protein-fragment complementation assay (PCA) based on the engineered
Deinococcus radiodurans infrared fluorescent protein IFP1. 4. Unlike previous fluorescent …

Protein–protein interactions (PPIs) orchestrate nearly all biological processes. They are also
considered attractive drug targets for treating many human diseases, including cancers and …

The low costs of array‐synthesized oligonucleotide libraries are empowering rapid
advances in quantitative and synthetic biology. However, high synthesis error rates, uneven …

Network biologists attempt to extract meaningful relationships among genes or their
products from very noisy data. We argue that what we categorize as noisy data may …

The identification of genomic variants in healthy and diseased individuals continues to
rapidly outpace our ability to functionally annotate these variants. Techniques that both …