qPCR-based methods for expression analysis of miRNAs

DA Forero, Y González-Giraldo, LJ Castro-Vega… - …, 2019 - Taylor & Francis
Several approaches for miRNA expression analysis have been developed in recent years.
In this article, we provide an updated and comprehensive review of available qPCR-based …

Plant reference genes for development and stress response studies

JT Joseph, NJ Poolakkalody, JM Shah - Journal of biosciences, 2018 - Springer
Many reference genes are used by different laboratories for gene expression analyses to
indicate the relative amount of input RNA/DNA in the experiment. These reference genes …

Identification of novel soybean microRNAs involved in abiotic and biotic stresses

FR Kulcheski, LFV de Oliveira, LG Molina, MP Almerão… - BMC genomics, 2011 - Springer
Abstract Background Small RNAs (19-24 nt) are key regulators of gene expression that
guide both transcriptional and post-transcriptional silencing mechanisms in eukaryotes …

Label-free platform for microRNA detection based on the fluorescence quenching of positively charged gold nanoparticles to silver nanoclusters

X Miao, Z Cheng, H Ma, Z Li, N Xue… - Analytical chemistry, 2018 - ACS Publications
A novel strategy was developed for microRNA-155 (miRNA-155) detection based on the
fluorescence quenching of positively charged gold nanoparticles [(+) AuNPs] to Ag …

Soybean miR172c Targets the Repressive AP2 Transcription Factor NNC1 to Activate ENOD40 Expression and Regulate Nodule Initiation

Y Wang, L Wang, Y Zou, L Chen, Z Cai, S Zhang… - The Plant …, 2014 - academic.oup.com
MicroRNAs are noncoding RNAs that act as master regulators to modulate various
biological processes by posttranscriptionally repressing their target genes. Repression of …

MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development

Y Wang, K Li, L Chen, Y Zou, H Liu, Y Tian, D Li… - Plant …, 2015 - academic.oup.com
Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis
with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for …

Implications of ethylene biosynthesis and signaling in soybean drought stress tolerance

FBM Arraes, MA Beneventi, ME Lisei de Sa… - BMC plant biology, 2015 - Springer
Background Ethylene is a phytohormone known for inducing a triple response in seedlings,
leaf abscission and other responses to various stresses. Several studies in model plants …

Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses

A Borges, SM Tsai, DGG Caldas - Plant cell reports, 2012 - Springer
Selection of reference genes is an essential consideration to increase the precision and
quality of relative expression analysis by the quantitative RT-PCR method. The stability of …

Ultrasensitive electrochemical detection of microRNA based on an arched probe mediated isothermal exponential amplification

Y Yu, Z Chen, L Shi, F Yang, J Pan, B Zhang… - Analytical …, 2014 - ACS Publications
In this work, a simple and label-free electrochemical biosensor is developed for microRNA
(miRNA) detection on the basis of an arched probe mediated isothermal exponential …

Electrochemiluminescence biosensor for miRNA-21 based on toehold-mediated strand displacement amplification with Ru (phen) 32+ loaded DNA nanoclews as …

Y Zhang, G Xu, G Lian, F Luo, Q Xie, Z Lin… - Biosensors and …, 2020 - Elsevier
A novel electrochemiluminescence (ECL) biosensor was developed for high sensitive and
selective detection of miRNA-21 based on the efficient and specific toehold-mediated strand …