Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …

Tripartite efflux pumps and the related type 1 secretion systems (T1SSs) in Gram-negative
organisms are diverse in function, energization, and structural organization. They form …

Membrane lipids interact with proteins in a variety of ways, ranging from providing a stable
membrane environment for proteins to being embedded in to detailed roles in complicated …

Oligomerization of membrane proteins in response to lipid binding has a critical role in many
cell-signalling pathways but is often difficult to define or predict. Here we report the …

The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has
ushered in a new age of promise and challenge for the characterization and identification of …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

The MacB ABC transporter forms a tripartite efflux pump with the MacA adaptor protein and
TolC outer membrane exit duct to expel antibiotics and export virulence factors from Gram …

Small molecules are known to stabilize membrane proteins and to modulate their function
and oligomeric state, but such interactions are often hard to precisely define. Here we …

Lipids tailor membrane identities and function as molecular hubs in all cellular processes.
However, the ways in which lipids modulate protein function and structure are poorly …

Now routine is the ability to investigate soluble and membrane protein complexes in the gas
phase of a mass spectrometer while preserving folded structure and ligand-binding …