Abstract The Replication Protein A (RPA) complex is an essential regulator of eukaryotic
DNA metabolism. RPA avidly binds to single-stranded DNA (ssDNA) through multiple …

Genome maintenance is a constant concern for cells, and a coordinated response to DNA
damage is required to maintain cellular viability and prevent disease. The ataxia …

The structural features that enable replicative DNA polymerases to synthesize DNA rapidly
and accurately also limit their ability to copy damaged DNA. Direct replication of DNA …

Mechanisms that preserve genome integrity are highly important during the normal life cycle
of human cells. Loss of genome protective mechanisms can lead to the development of …

The heterotrimeric eukaryotic Replication protein A (RPA) is a master regulator of numerous
DNA metabolic processes. For a long time, it has been viewed as an inert protector of …

To maintain genome stability, cells have evolved various DNA repair pathways to deal with
oxidative DNA damage. DNA damage response (DDR) pathways, including ATM-Chk2 and …

Kinases in the phosphoinositide three-kinase-related kinase (PIKK) family include ATM
(ataxia-telangiectasia mutated), ATR (ATM-and Rad3-related), DNA-PKcs (DNA-dependent …

TopBP1 serves as an activator of the ATR-ATRIP complex in response to the presence of
incompletely replicated or damaged DNA. This process involves binding of ATR to the ATR …

Here, we demonstrate that primed, single-stranded DNA (ssDNA) is sufficient for activation
of the ATR-dependent checkpoint pathway in Xenopus egg extracts. Using this structure, we …

Since the initial discovery of replication protein A (RPA) as a DNA replication factor, much
progress has been made on elucidating critical roles for RPA in other DNA metabolic …