Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis

TM Porter, M Hajibabaei - Molecular ecology, 2018 - Wiley Online Library
The purpose of this review is to present the most common and emerging DNA‐based
methods used to generate data for biodiversity and biomonitoring studies. As environmental …

A new way to contemplate Darwin's tangled bank: how DNA barcodes are reconnecting biodiversity science and biomonitoring

M Hajibabaei, DJ Baird, NA Fahner… - … of the Royal …, 2016 - royalsocietypublishing.org
Encompassing the breadth of biodiversity in biomonitoring programmes has been frustrated
by an inability to simultaneously identify large numbers of species accurately and in a timely …

Over 2.5 million COI sequences in GenBank and growing

TM Porter, M Hajibabaei - PloS one, 2018 - journals.plos.org
The increasing popularity of cytochrome c oxidase subunit 1 (COI) DNA metabarcoding
warrants a careful look at the underlying reference databases used to make high-throughput …

Automated high throughput animal CO1 metabarcode classification

TM Porter, M Hajibabaei - Scientific Reports, 2018 - nature.com
We introduce a method for assigning names to CO1 metabarcode sequences with
confidence scores in a rapid, high-throughput manner. We compiled nearly 1 million CO1 …

Peering into the darkness: DNA barcoding reveals surprisingly high diversity of unknown species of Diptera (Insecta) in Germany

C Chimeno, A Hausmann, S Schmidt, MJ Raupach… - Insects, 2022 - mdpi.com
Simple Summary Roughly two-thirds of the insect species described from Germany belong
to the orders Diptera (flies) or Hymenoptera (wasps, bees, ants and sawflies). However, both …

Testing the potential of a ribosomal 16S marker for DNA metabarcoding of insects

V Elbrecht, P Taberlet, T Dejean, A Valentini… - PeerJ, 2016 - peerj.com
Cytochrome c oxidase I (COI) is a powerful marker for DNA barcoding of animals, with good
taxonomic resolution and a large reference database. However, when used for DNA …

COI metabarcoding primer choice affects richness and recovery of indicator taxa in freshwater systems

M Hajibabaei, TM Porter, M Wright, J Rudar - PLoS One, 2019 - journals.plos.org
Mixed community or environmental DNA marker gene sequencing has become a commonly
used technique for biodiversity analyses in freshwater systems. Many cytochrome c oxidase …

Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches

J Batovska, AM Piper, I Valenzuela, JP Cunningham… - Scientific Reports, 2021 - nature.com
Metabarcoding has the potential to revolutionise insect surveillance by providing high-
throughput and cost-effective species identification of all specimens within mixed trap …

Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes

B Günther, T Knebelsberger, H Neumann… - Scientific Reports, 2018 - nature.com
We establish the new approach of environmental DNA (eDNA) analyses for the North Sea.
Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase …

Species from feces: order-wide identification of Chiroptera from guano and other non-invasive genetic samples

FM Walker, CHD Williamson, DE Sanchez, CJ Sobek… - Plos one, 2016 - journals.plos.org
Bat guano is a relatively untapped reservoir of information, having great utility as a DNA
source because it is often available at roosts even when bats are not and is an easy type of …