The common and persistent failures to translate promising preclinical drug candidates into
clinical success highlight the limited effectiveness of disease models currently used in drug …

Current research in biology uses evermore complex computational and imaging tools. Here
we describe Icy, a collaborative bioimage informatics platform that combines a community …

Background ImageJ is an image analysis program extensively used in the biological
sciences and beyond. Due to its ease of use, recordable macro language, and extensible …

Vimentin has been shown to be involved in wound healing, but its functional contribution to
this process is poorly understood. Here we describe a previously unrecognized function of …

Abstract RNA-sequencing (RNA-seq) measures the quantitative change in gene expression
over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization …

Understanding the structure–function relationship of cells and organelles in their natural
context requires multidimensional imaging. As techniques for multimodal 3-D imaging have …

Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …

Few technologies are more widespread in modern biological laboratories than imaging.
Recent advances in optical technologies and instrumentation are providing hitherto …

Single nucleus segmentation is a frequent challenge of microscopy image processing, since
it is the first step of many quantitative data analysis pipelines. The quality of tracking single …

High-content screening (HCS), which combines automated fluorescence microscopy with
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …