Transcriptome studies reveal pervasive transcription of complex genomes, such as those of
mammals. Despite popular arguments for functionality of most, if not all, of these transcripts …

Alternative splicing of eukaryotic transcripts is a mechanism that enables cells to generate
vast protein diversity from a limited number of genes. The mechanisms and outcomes of …

Precursor messenger RNA (pre-mRNA) splicing is a critical step in the posttranscriptional
regulation of gene expression, providing significant expansion of the functional proteome of …

Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA
polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the …

The 3′ end of most protein-coding genes and long non-coding RNAs is cleaved and
polyadenylated. Recent discoveries have revealed that a large proportion of these genes …

Deep sequencing of embryonic stem cell RNA revealed many specific internal introns that
are significantly more abundant than the other introns within polyadenylated transcripts; we …

Alternative polyadenylation (APA) is emerging as a widespread mechanism used to control
gene expression. Like alternative splicing, usage of alternative poly (A) sites allows a single …

Serine and arginine-rich (SR) proteins are RNA-binding proteins (RBPs) known as
constitutive and alternative splicing regulators. As splicing is linked to transcriptional and …

Elongation is becoming increasingly recognized as a critical step in eukaryotic
transcriptional regulation. Although traditional genetic and biochemical studies have …

The Mediator complex is a multi-subunit assembly that appears to be required for regulating
expression of most RNA polymerase II (pol II) transcripts, which include protein-coding and …