The active form of the small GTPase RhoA is necessary and sufficient for formation of a
cytokinetic furrow in animal cells. Despite the conceptual simplicity of the process, the …

Structural biology is currently undergoing a transformation into dynamic structural biology,
which reveals the dynamic structure of proteins during their functional activity to better …

Optical super-resolution imaging with structured illumination microscopy (SIM) is a key
technology for the visualization of processes at the molecular level in the chemical and …

High-speed atomic force microscopy (HS-AFM) is a unique tool for molecular imaging. It can
directly visualize protein molecules during their functional activity at high spatiotemporal …

In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and
chromosome segregation–essential to prevent miscarriage or developmental defects–thus …

MKLP2, a kinesin-6, has critical roles during the metaphase-anaphase transition and
cytokinesis. Its motor domain contains conserved nucleotide binding motifs, but is divergent …

Centralspindlin, a complex of the MKLP1 kinesin-6 and CYK4 GAP subunits, plays key roles
in metazoan cytokinesis. CYK4-binding to the long neck region of MKLP1 restricts the …

Contractile ring constriction during cytokinesis is thought to compact central spindle
microtubules to form the midbody, an antiparallel microtubule bundle at the intercellular …

Cleavage furrow in animal cell cytokinesis is formed by cortical constriction driven by
contraction of an actomyosin network activated by Rho GTPase. Although the role of the …

Kinesin motor domains generate impulses of force and movement that have both
translational and rotational (torque) components. Here, we ask how the torque component …