Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Technologies and standardization in research on extracellular vesicles

S Gandham, X Su, J Wood, AL Nocera, SC Alli… - Trends in …, 2020 - cell.com
Extracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures
containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various …

The role of exosomes in liquid biopsy for cancer diagnosis and prognosis prediction

S Li, M Yi, B Dong, X Tan, S Luo… - International journal of …, 2021 - Wiley Online Library
Liquid biopsy is a revolutionary strategy in cancer diagnosis and prognosis prediction, which
is used to analyze cancer cells or cancer‐derived products through biofluids such as blood …

[HTML][HTML] From genome-wide arrays to tailor-made biomarker readout–Progress towards routine analysis of skin sensitizing chemicals with GARD

A Forreryd, KS Zeller, T Lindberg, H Johansson… - Toxicology in Vitro, 2016 - Elsevier
Allergic contact dermatitis (ACD) initiated by chemical sensitizers is an important public
health concern. To prevent ACD, it is important to identify chemical allergens to limit the use …

[HTML][HTML] Modelling of compound combination effects and applications to efficacy and toxicity: state-of-the-art, challenges and perspectives

KC Bulusu, R Guha, DJ Mason, RPI Lewis… - Drug discovery today, 2016 - Elsevier
Highlights•Review of the state-of-the-art in the field of compound combination modelling.•
Significance of quality control of large-scale combination screening data.•Strategies for …

Nanoliter high throughput quantitative PCR

T Morrison, J Hurley, J Garcia, K Yoder… - Nucleic acids …, 2006 - academic.oup.com
Understanding biological complexity arising from patterns of gene expression requires
accurate and precise measurement of RNA levels across large numbers of genes …

Identification of AbrB‐regulated genes involved in biofilm formation by Bacillus subtilis

MA Hamon, NR Stanley, RA Britton… - Molecular …, 2004 - Wiley Online Library
Bacillus subtilis is a ubiquitous soil bacterium that forms biofilms in a process that is
negatively controlled by the transcription factor AbrB. To identify the AbrB‐regulated genes …

Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of …

JW Shin, R Huggenberger… - Blood, The Journal of the …, 2008 - ashpublications.org
Lymphatic vessel growth and activation, mediated by vascular endothelial growth factor
(VEGF)–C and/or VEGF-A, have important roles in metastasis and in chronic inflammation …

Methodologies to decipher the cell secretome

P Mukherjee, S Mani - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2013 - Elsevier
The cell secretome is a collection of proteins consisting of transmembrane proteins (TM) and
proteins secreted by cells into the extracellular space. A significant portion (~ 13–20%) of the …

Loss of gene information: Discrepancies between RNA sequencing, cDNA microarray, and qRT-PCR

N Rachinger, S Fischer, I Böhme… - International Journal of …, 2021 - mdpi.com
Molecular analyses of normal and diseased cells give insight into changes in gene
expression and help in understanding the background of pathophysiological processes …