We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED),
as discussed during a symposium at the National Center for CryoEM Access and Training …

Structural genomics consortia established that protein crystallization is the primary obstacle
to structure determination using x‐ray crystallography. We previously demonstrated that …

Microcrystal electron diffraction (MicroED) is an emerging structural technique in which
submicron crystals are used to generate diffraction data for structural studies. Structures …

Nonribosomal peptide synthetases (NRPSs) are responsible for the production of important
biologically active peptides. The large, multidomain NRPSs operate through an assembly …

X-ray crystallography is the most commonly employed technique to discern macromolecular
structures, but the crucial step of crystallizing a protein into an ordered lattice amenable to …

Imaging scaffolds composed of designed protein cages fused to designed ankyrin repeat
proteins (DARPins) have enabled the structure determination of small proteins by cryogenic …

Protein crystallography is an established method to study the atomic structures of
macromolecules and their complexes. A prerequisite for successful structure determination …

Protocols for robotic protein crystallization using the Crystallization Facility at Harwell and in
situ room temperature data collection from crystallization plates at Diamond Light Source …

X-ray crystallography has for most of the last century been the standard technique to
determine the high-resolution structure of biological macromolecules, including multi …

Enzymes have evolved to rapidly and selectively hydrolyze diverse natural and
anthropogenic polymers, but only a limited group of related enzymes have been shown to …