The technological landscape and applications of single-cell multi-omics

A Baysoy, Z Bai, R Satija, R Fan - Nature Reviews Molecular Cell …, 2023 - nature.com
Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

Single-cell proteomics enabled by next-generation sequencing or mass spectrometry

HM Bennett, W Stephenson, CM Rose, S Darmanis - Nature Methods, 2023 - nature.com
In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

CODEX multiplexed tissue imaging with DNA-conjugated antibodies

S Black, D Phillips, JW Hickey, J Kennedy-Darling… - Nature protocols, 2021 - nature.com
Advances in multiplexed imaging technologies have drastically improved our ability to
characterize healthy and diseased tissues at the single-cell level. Co-detection by indexing …

Single-cell multiomics: technologies and data analysis methods

J Lee, DY Hyeon, D Hwang - Experimental & Molecular Medicine, 2020 - nature.com
Advances in single-cell isolation and barcoding technologies offer unprecedented
opportunities to profile DNA, mRNA, and proteins at a single-cell resolution. Recently, bulk …

Spatial imaging of glycoRNA in single cells with ARPLA

Y Ma, W Guo, Q Mou, X Shao, M Lyu, V Garcia… - Nature …, 2024 - nature.com
Little is known about the biological roles of glycosylated RNAs (glycoRNAs), a recently
discovered class of glycosylated molecules, because of a lack of visualization methods. We …

Applications of multi‐omics analysis in human diseases

C Chen, J Wang, D Pan, X Wang, Y Xu, J Yan… - MedComm, 2023 - Wiley Online Library
Multi‐omics usually refers to the crossover application of multiple high‐throughput screening
technologies represented by genomics, transcriptomics, single‐cell transcriptomics …

Simultaneous epitope and transcriptome measurement in single cells

M Stoeckius, C Hafemeister, W Stephenson… - Nature …, 2017 - nature.com
High-throughput single-cell RNA sequencing has transformed our understanding of complex
cell populations, but it does not provide phenotypic information such as cell-surface protein …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Machine learning for integrating data in biology and medicine: Principles, practice, and opportunities

M Zitnik, F Nguyen, B Wang, J Leskovec… - Information …, 2019 - Elsevier
New technologies have enabled the investigation of biology and human health at an
unprecedented scale and in multiple dimensions. These dimensions include a myriad of …

Higher-order inter-chromosomal hubs shape 3D genome organization in the nucleus

SA Quinodoz, N Ollikainen, B Tabak, A Palla… - Cell, 2018 - cell.com
Eukaryotic genomes are packaged into a 3-dimensional structure in the nucleus. Current
methods for studying genome-wide structure are based on proximity ligation. However, this …