Transformation-associated recombination (TAR) cloning represents a unique tool for
isolation and manipulation of large DNA molecules. The technique exploits a high level of …

The basic components of the plant cell cycle are G1 (postmitotic interphase), S‐phase (DNA
synthesis phase), G2 (premitotic interphase) and mitosis/cytokinesis. Proliferating cells are …

We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations.
The system is based on an auxotrophic mutant host of P. pastoris which is defective in …

Fragments of exogenous DNA that range in size up to several hundred kilobase pairs have
been cloned into yeast by ligating them to vector sequences that allow their propagation as …

Saccharomyces teiomeres consist of-300 bp of C,. & TGlms DNA. Nondenaturing Southern
hybridization, capable of detecting-60 to-300 bases of TGl. a DNA, revealed that yeast …

Yeast is one of the oldest domesticated organisms and has both industrial and domestic
applications. In addition, it is very widely used as a eukaryotic model organism in biological …

The phrase'gene manipulation'means different things to different people. Some, for example,
would consider the sophisticated genetic manipulations undertaken daily by Escherichia coli …

DNA fragments that function as autonomously replicating sequences (ARSs) have been
isolated from Ustilago maydis. When inserted into an integrative transforming vector, the …

A colony color assay that measures chromosome stability is described and is used to study
several parameters affecting the mitotic maintenance of yeast chromosomes, including ARS …

We have used pediiree analysis to investigate the mitotic segregation of circular and linear
DNA plasmids in Saccharomyces cerevisae. Circular ARS plasmids, which bear putative …