Despite the surge of microbial genome data, experimental testing is important to confirm
inferences about the cell biology, ecological roles and evolution of microorganisms. As the …

Corals associate not only with dinoflagellates, which are their algal endosymbionts and
which have been extensively studied over the past four decades, but also with a variety of …

Wetland soils are the greatest source of nitrous oxide (N2O), a critical greenhouse gas and
ozone depleter released by microbes. Yet, microbial players and processes underlying the …

Nature conservation literature and policy instruments mainly focus on the impacts of human
development and the benefits of nature conservation for oceans and aboveground terrestrial …

Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried
out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing …

Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA)
genes has been an approach widely used in environmental microbiology, particularly since …

Plastic debris in the ocean form a new ecosystem, termed 'plastisphere', which hosts a
variety of marine organisms. Recent studies implemented DNA metabarcoding to …

Commonly used 16S rRNA gene primers do not detect the full range of archaeal diversity
present in the vertebrate gut. As a result, several questions regarding the archaeal …

Rare biosphere represents the majority of Earth's biodiversity and performs vital ecological
functions, yet little is known about its biogeographical patterns and community assembly …