Digital twins for tissue culture techniques—concepts, expectations, and state of the art

J Möller, R Pörtner - Processes, 2021 - mdpi.com
Techniques to provide in vitro tissue culture have undergone significant changes during the
last decades, and current applications involve interactions of cells and organoids, three …

Design, fabrication, and characterization of a multimodal reconfigurable bioreactor for bone tissue engineering

M Montorsi, GG Genchi, D De Pasquale… - Biotechnology and …, 2022 - Wiley Online Library
In the past decades, bone tissue engineering developed and exploited many typologies of
bioreactors, which, besides providing proper culture conditions, aimed at integrating those …

Computer-aided engineering and additive manufacturing for bioreactors in tissue engineering: State of the art and perspectives

GM Di Gravina, G Loi, F Auricchio, M Conti - Biophysics Reviews, 2023 - pubs.aip.org
Two main challenges are currently present in the healthcare world, ie, the limitations given
by transplantation and the need to have available 3D in vitro models. In this context …

Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols

JC Silva, J Meneses, FFF Garrudo, SR Fernandes… - Scientific Reports, 2024 - nature.com
Electrical stimulation (ES) has been described as a promising tool for bone tissue
engineering, being known to promote vital cellular processes such as cell proliferation …

JANUS: an open-source 3D printable perfusion bioreactor and numerical model-based design strategy for tissue engineering

J Meneses, SR Fernandes, JC Silva… - … in Bioengineering and …, 2023 - frontiersin.org
Bioreactors have been employed in tissue engineering to sustain longer and larger cell
cultures, managing nutrient transfer and waste removal. Multiple designs have been …

Medicated multi-targeted alginate-based dressings for potential treatment of mixed bacterial-fungal infections in diabetic foot ulcers

A Ahmed, G Getti, J Boateng - International journal of pharmaceutics, 2021 - Elsevier
Recently developed medicated dressings target either bacterial or fungal infection only,
which is not effective for the treatment of mixed infections common in diabetic foot ulcers …

CyMAD bioreactor: A cyclic magnetic actuation device for magnetically mediated mechanical stimulation of 3D bioprinted hydrogel scaffolds

C Czichy, D Kilian, TC Wang, S Günther, A Lode… - Journal of the …, 2022 - Elsevier
Mechanical stimulation of bioprinted constructs can enhance the differentiation of cells
within these scaffolds, such as driving chondrocytes towards cartilage tissue substitutes. In …

Synergy between 3D-extruded electroconductive scaffolds and electrical stimulation to improve bone tissue engineering strategies

JC Silva, P Marcelino, J Meneses, F Barbosa… - Journal of Materials …, 2024 - pubs.rsc.org
In this work, we propose a simple, reliable, and versatile strategy to create 3D
electroconductive scaffolds suitable for bone tissue engineering (TE) applications with …

Computational and experimental investigation of the combined effect of various 3d scaffolds and bioreactor stimulation on human cells' feedback

FK Kozaniti, AE Manara, V Kostopoulos, P Mallis… - Applied …, 2023 - mdpi.com
Computational methods were combined with an experimental setup in order to investigate
the response of human umbilical cord stem cells to 3D electrospun and printed scaffolds …

A novel approach for design and manufacturing of curvature-featuring scaffolds for osteochondral repair

P Marcelino, JC Silva, CS Moura, J Meneses… - Polymers, 2023 - mdpi.com
Osteochondral (OC) defects affect both articular cartilage and the underlying subchondral
bone. Due to limitations in the cartilage tissue's self-healing capabilities, OC defects exhibit …