Site-selective modification strategies in antibody–drug conjugates

SJ Walsh, JD Bargh, FM Dannheim… - Chemical Society …, 2021 - pubs.rsc.org
Antibody–drug conjugates (ADCs) harness the highly specific targeting capabilities of an
antibody to deliver a cytotoxic payload to specific cell types. They have garnered …

Contemporary Approaches for Site‐Selective Dual Functionalization of Proteins

L Xu, SL Kuan, T Weil - Angewandte Chemie International …, 2021 - Wiley Online Library
Site‐selective protein functionalization serves as an invaluable tool for investigating protein
structures and functions in complicated cellular environments and accomplishing semi …

Platform for Orthogonal N-Cysteine-Specific Protein Modification Enabled by Cyclopropenone Reagents

A Istrate, MB Geeson, CD Navo, BB Sousa… - Journal of the …, 2022 - ACS Publications
Protein conjugates are valuable tools for studying biological processes or producing
therapeutics, such as antibody–drug conjugates. Despite the development of several protein …

Site-specific bioconjugation approaches for enhanced delivery of protein therapeutics and protein drug carriers

RM Lieser, D Yur, MO Sullivan, W Chen - Bioconjugate chemistry, 2020 - ACS Publications
Proteins have the capacity to treat a multitude of diseases both as therapeutics and as drug
carriers due to their complex functional properties, specificity toward binding partners …

Protein engineering strategies for improved pharmacokinetics

A Rondon, S Mahri, F Morales‐Yanez… - Advanced Functional …, 2021 - Wiley Online Library
Protein therapeutics have gained momentum in recent years and become a pillar in treating
many diseases and the only choice in several ailments. Protein therapeutics are highly …

Harnessing sortase A transpeptidation for advanced targeted therapeutics and vaccine engineering

EM Obeng, AJ Fulcher, KM Wagstaff - Biotechnology Advances, 2023 - Elsevier
The engineering of potent prophylactic and therapeutic complexes has always required
careful protein modification techniques with seamless capabilities. In this light, methods that …

Photofunctional cyclometalated iridium (III) polypyridine complexes bearing a perfluorobiphenyl moiety for bioconjugation, bioimaging, and phototherapeutic …

LCC Lee, AWY Tsang, HW Liu, KKW Lo - Inorganic Chemistry, 2020 - ACS Publications
In this article, we report the design, synthesis, and characterization of a series of
cyclometalated iridium (III) polypyridine complexes containing a perfluorobiphenyl (PFBP) …

π-Clamp-mediated homo-and heterodimerization of single-domain antibodies via site-specific homobifunctional conjugation

RJ Taylor, M Aguilar Rangel, MB Geeson… - Journal of the …, 2022 - ACS Publications
Post-translational protein–protein conjugation produces bioconjugates that are unavailable
via genetic fusion approaches. A method for preparing protein–protein conjugates using π …

Site-specific dual-labeling of a VHH with a chelator and a photosensitizer for nuclear imaging and targeted photodynamic therapy of EGFR-positive tumors

E Renard, E Collado Camps, C Canovas, A Kip… - Cancers, 2021 - mdpi.com
Simple Summary Variable domains of heavy chain only antibodies are small proteins that
can be used for tumor imaging and therapy upon conjugation of functional groups. As …

Dual site-specific chemoenzymatic antibody fragment conjugation using CRISPR-based hybridoma engineering

CM Le Gall, JMS van der Schoot… - Bioconjugate …, 2021 - ACS Publications
Functionalized antibodies and antibody fragments have found applications in the fields of
biomedical imaging, theranostics, and antibody–drug conjugates (ADC). In addition …