Research progress of multi-enzyme complexes based on the design of scaffold protein

X Wang, Y Jiang, H Liu, H Yuan, D Huang… - Bioresources and …, 2023 - Springer
Multi-enzyme complexes designed based on scaffold proteins are a current topic in
molecular enzyme engineering. They have been gradually applied to increase the …

Current challenges in designer cellulosome engineering

B Lamote, MJM da Fonseca, J Vanderstraeten… - Applied Microbiology …, 2023 - Springer
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising
carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high …

Engineering an artificial catch bond using mechanical anisotropy

Z Liu, H Liu, AM Vera, B Yang, P Tinnefeld… - Nature …, 2024 - nature.com
Catch bonds are a rare class of protein-protein interactions where the bond lifetime
increases under an external pulling force. Here, we report how modification of anchor …

Single-molecule dynamic structural biology with vertically arranged DNA on a fluorescence microscope

AM Szalai, G Ferrari, L Richter, J Hartmann, MZ Kesici… - Nature …, 2024 - nature.com
The intricate interplay between DNA and proteins is key for biological functions such as DNA
replication, transcription and repair. Dynamic nanoscale observations of DNA structural …

A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome

M Duarte, A Viegas, VD Alves, JAM Prates… - Journal of Biological …, 2021 - ASBMB
The Cellulosome is an intricate macromolecular protein complex that centralizes the
cellulolytic efforts of many anaerobic microorganisms through the promotion of enzyme …

Recent advances in bioinspired multienzyme engineering for food applications

X Chen, Y Chen, D Tang, M Li, Y Lu, Y Cao… - Trends in Food Science …, 2024 - Elsevier
Background Bioinspired multienzyme engineering provides strong technical support for
enhancing food quality, safety, and nutrition. Summarizing and examining its applications …

In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein

X Wang, Y Jiang, H Liu, X Zhang, H Yuan… - … in Bioengineering and …, 2023 - frontiersin.org
Introduction: Trehalose is a significant rare sugar known for its stable properties and ability
to protect biomolecules from environmental factors. Methods: In this study, we present a …

[HTML][HTML] Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies

M Duarte, VD Alves, M Correia, C Caseiro… - International journal of …, 2023 - Elsevier
The cellulosome is an elaborate multi-enzyme structure secreted by many anaerobic
microorganisms for the efficient degradation of lignocellulosic substrates. It is composed of …

A simple and general approach to generate photoactivatable DNA processing enzymes

MZ Kesici, P Tinnefeld, AM Vera - Nucleic Acids Research, 2022 - academic.oup.com
DNA processing enzymes, such as DNA polymerases and endonucleases, have found
many applications in biotechnology, molecular diagnostics, and synthetic biology, among …

Protein engineering approach to enhance activity assays of mono-ADP-ribosyltransferases through proximity

A Galera-Prat, J Alaviuhkola, HI Alanen… - … , Design and Selection, 2022 - academic.oup.com
Human mono-ADP-ribosylating PARP enzymes have been linked to several clinically
relevant processes and many of these PARPs have been suggested as potential drug …