Blood samples are widely used for PCR-based DNA analysis in fields such as diagnosis of
infectious diseases, cancer diagnostics, and forensic genetics. In this study, the mechanisms …

Foodborne and enteric viruses continue to impose a significant public health and economic
burden globally. As many of these viruses are highly transmissible, the ability to detect them …

Inhibition of PCR by metal ions can pose a serious challenge in the process of forensic DNA
analysis. Samples contaminated with various types of metal ions encountered at crime …

Cattle are considered to be the main reservoir for Shiga toxin-producing Escherichia coli
(STEC) and are often the direct or indirect source of STEC outbreaks in humans. Accurate …

Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform
assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is …

DNA quantification is a vital step in forensic DNA analysis to determine the optimal input
amount for DNA typing. A quantitative real-time polymerase chain reaction (qPCR) assay …

Insertion/deletion polymorphisms (InDels) have particular characteristics, such as a
relatively low mutation rate, small amplicon size, and no stutter artifacts when genotyped via …

The goal of this study was to develop a method for the detection of semen in biological
stains using high-resolution melt (HRM) analysis and DNA methylation. To perform this task …

The assessment of degradation is crucial for the analysis of human DNA samples isolated
from forensic specimens. Forensic quantitative PCR (qPCR) assays can include multiple …

Human remains can be severely affected by the environment, and the DNA may be
damaged, degraded, and/or inhibited. In this study, a DNA sample (at 1 ng DNA target input …