Exosomes are small, single-membrane, secreted organelles of∼ 30 to∼ 200 nm in
diameter that have the same topology as the cell and are enriched in selected proteins …

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has
revolutionized the field of gene editing. Continuous efforts in developing this technology …

Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may
cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is …

In a mature, infectious HIV-1 virion, the viral genome is housed within a conical capsid core
made from the viral capsid (CA) protein. The CA protein and the structure into which it …

INTRODUCTION Reverse transcription and integration are the signature events of retrovirus
replication. Reverse transcription creates a double-stranded DNA (dsDNA) copy of the …

HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization,
and mechanism of uncoating are under debate. We adapted a strategy to visualize …

Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian
cells and are now commonly utilised within the growing field of cell and gene therapy for the …

Physical Biology of the Cell is a textbook for a first course in physical biology or biophysics
for undergraduate or graduate students. It maps the huge and complex landscape of cell …

The HIV-1 core consists of capsid proteins (CA) surrounding viral genomic RNA. After virus-
cell fusion, the core enters the cytoplasm and the capsid shell is lost through uncoating. CA …

Selection of T-cell vaccine antigens for chronic persistent viral infections has been largely
empirical. To define the relationship, at the population level, between the specificity of the …