The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

The fidelity of intracellular signaling hinges on the organization of dynamic activity
architectures. Spatial compartmentation was first proposed over 30 years ago to explain how …

Both mitochondria and lysosomes are essential for maintaining cellular homeostasis, and
dysfunction of both organelles has been observed in multiple diseases,,,. Mitochondria are …

Comprehensively resolving neuronal identities in whole-brain images is a major challenge.
We achieve this in C. elegans by engineering a multicolor transgene called NeuroPAL (a …

Elevated reactive oxygen species (ROS) induce the formation of lipids in neurons that are
transferred to glia, where they form lipid droplets (LDs). We show that glial and neuronal …

The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …

We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a
tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum …

How sublethal levels of antibiotics and heavy metals select for clinically important multidrug
resistance plasmids is largely unknown. Carriage of plasmids generally confers substantial …

Monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein
tags and components of biosensors for deep-tissue imaging and multicolour microscopy. We …