Expansion microscopy: A chemical approach for super-resolution microscopy
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …
Multiplex protein imaging in tumour biology
N de Souza, S Zhao, B Bodenmiller - Nature Reviews Cancer, 2024 - nature.com
Tissue imaging has become much more colourful in the past decade. Advances in both
experimental and analytical methods now make it possible to image protein markers in …
experimental and analytical methods now make it possible to image protein markers in …
Single-shot 20-fold expansion microscopy
Expansion microscopy (ExM) is in increasingly widespread use throughout biology because
its isotropic physical magnification enables nanoimaging on conventional microscopes. To …
its isotropic physical magnification enables nanoimaging on conventional microscopes. To …
Light-microscopy based dense connectomic reconstruction of mammalian brain tissue
The information-processing capability of the brain's cellular network depends on the
physical wiring pattern between neurons and their molecular and functional characteristics …
physical wiring pattern between neurons and their molecular and functional characteristics …
Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues
A Glaser, J Chandrashekar, S Vasquez, C Arshadi… - bioRxiv, 2023 - biorxiv.org
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing
unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and …
unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and …
Expanding boundaries–a cell biologist's guide to expansion microscopy
N Hümpfer, R Thielhorn, H Ewers - Journal of Cell Science, 2024 - journals.biologists.com
Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in
light microscopy. In contrast to super-resolution microscopy methods that rely on …
light microscopy. In contrast to super-resolution microscopy methods that rely on …
[HTML][HTML] Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues
Lipid membranes are key to the nanoscale compartmentalization of biological systems, but
fluorescent visualization of them in intact tissues, with nanoscale precision, is challenging to …
fluorescent visualization of them in intact tissues, with nanoscale precision, is challenging to …
Coaching ribosome biogenesis from the nuclear periphery
Severe invagination of the nuclear envelope is a hallmark of cancers, aging,
neurodegeneration, and infections. However, the outcomes of nuclear invagination remain …
neurodegeneration, and infections. However, the outcomes of nuclear invagination remain …
autoFISH-a modular toolbox for sequential smFISH experiments
C Weber, T Defard, M Lelek, H Laporte, A Mallick… - bioRxiv, 2024 - biorxiv.org
Fluorescence in situ hybridization (FISH) allows for spatial and quantitative profiling of gene
expression by visualizing individual RNA molecules. Here, we introduce automated FISH …
expression by visualizing individual RNA molecules. Here, we introduce automated FISH …