Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Multiplex protein imaging in tumour biology

N de Souza, S Zhao, B Bodenmiller - Nature Reviews Cancer, 2024 - nature.com
Tissue imaging has become much more colourful in the past decade. Advances in both
experimental and analytical methods now make it possible to image protein markers in …

Single-shot 20-fold expansion microscopy

S Wang, TW Shin, HB Yoder, RB McMillan, H Su… - Nature …, 2024 - nature.com
Expansion microscopy (ExM) is in increasingly widespread use throughout biology because
its isotropic physical magnification enables nanoimaging on conventional microscopes. To …

Light-microscopy based dense connectomic reconstruction of mammalian brain tissue

MR Tavakoli, J Lyudchik, M Januszewski, V Vistunou… - bioRxiv, 2024 - biorxiv.org
The information-processing capability of the brain's cellular network depends on the
physical wiring pattern between neurons and their molecular and functional characteristics …

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues

A Glaser, J Chandrashekar, S Vasquez, C Arshadi… - bioRxiv, 2023 - biorxiv.org
Recent advances in tissue processing, labeling, and fluorescence microscopy are providing
unprecedented views of the structure of cells and tissues at sub-diffraction resolutions and …

Expanding boundaries–a cell biologist's guide to expansion microscopy

N Hümpfer, R Thielhorn, H Ewers - Journal of Cell Science, 2024 - journals.biologists.com
Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in
light microscopy. In contrast to super-resolution microscopy methods that rely on …

[HTML][HTML] Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues

TW Shin, H Wang, C Zhang, B An, Y Lu, E Zhang, X Lu… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Lipid membranes are key to the nanoscale compartmentalization of biological systems, but
fluorescent visualization of them in intact tissues, with nanoscale precision, is challenging to …

Coaching ribosome biogenesis from the nuclear periphery

Y Zhuang, X Guo, OV Razorenova, CE Miles… - …, 2024 - pmc.ncbi.nlm.nih.gov
Severe invagination of the nuclear envelope is a hallmark of cancers, aging,
neurodegeneration, and infections. However, the outcomes of nuclear invagination remain …

autoFISH-a modular toolbox for sequential smFISH experiments

C Weber, T Defard, M Lelek, H Laporte, A Mallick… - bioRxiv, 2024 - biorxiv.org
Fluorescence in situ hybridization (FISH) allows for spatial and quantitative profiling of gene
expression by visualizing individual RNA molecules. Here, we introduce automated FISH …