Glycoconjugates are the ubiquitous components of mammalian cells, mainly synthesized by
covalent bonds of carbohydrates to other biomolecules such as proteins and lipids, with a …

Abstract Background Human milk oligosaccharides (HMOs) are a group of structurally
diverse unconjugated carbohydrates that are bioactive and functional beyond their important …

Glycosylation is a successful strategy to alter the pharmacological properties of small
molecules, and it has emerged as a unique approach to expand the chemical space of …

Abstract Glycoside hydrolase family 29 (GH29) encompasses α-L-fucosidases, ie enzymes
that catalyze the hydrolytic release of fucose from fucosylated glycans, including N-and O …

Highlights•Glycoside hydrolases can be converted into proficient transglycosidases.•It is
possible to switch the bond-type specificity of O/C/N/S-glycosyltransferases.•Glycoside …

Identifying function-enhancing enzyme variants is a 'holy grail'challenge in protein science
because it will allow researchers to expand the biocatalytic toolbox for late-stage …

Amylomaltases (AMs) play important roles in glycogen and maltose metabolism. However,
the molecular mechanism is elusive. Here, we investigated the conformational dynamics of …

Glycosides are widely used in many fields due to their favorable biological activity. The
traditional plant extractions and chemical methods for glycosides production are limited by …

Residues beyond the first coordination shell are often observed to make considerable
cumulative contributions in enzymes. Due to typically indirect perturbations of multiple …

Enzymatic synthesis is an elegant biocompatible approach to complex compounds such as
human milk oligosaccharides (HMOs). These compounds are vital for healthy neonatal …