Cyclic guanidines are found in many biologically active compounds and natural products.
Further, the formation of the atypical seven-membered ring of cyclic guanidine remains …

Pyruvate carboxylase is a metabolic enzyme that fuels the tricarboxylic acid cycle with one of
its intermediates and also participates in the first step of gluconeogenesis. This large …

Allosteric regulation of the essential anaplerotic enzyme, pyruvate carboxylase (PC), is vital
for metabolic homeostasis. PC catalyzes the bicarbonate-and ATP-dependent carboxylation …

Biotin-dependent enzymes catalyze carboxyl transfer reactions by efficiently coordinating
multiple reactions between spatially distinct active sites. Pyruvate carboxylase (PC), a …

Allosteric regulation of pyruvate carboxylase (PC) activity is pivotal to maintaining metabolic
homeostasis. In contrast, dysregulated PC activity contributes to the pathogenesis of …

Protein structure, ligand binding, and catalytic turnover contributes to the governance of
catalytic events occurring at spatially distinct domains in multifunctional enzymes …

l-Aspartate is a regulatory feedback inhibitor of the biotin-dependent enzyme pyruvate
carboxylase in response to increased levels of tricarboxylic acid cycle intermediates …

The mechanism of allosteric activation of pyruvate carboxylase by acetyl CoA is not fully
understood. Here we have examined the roles of residues near the acetyl CoA binding site …

A previous study showed that 2′-3′-O-(2, 4, 6-trinitrophenyl) adenosine 5′-triphosphate
(TNP-ATP) was a weak allosteric activator of Rhizobium etli pyruvate carboxylase (RePC) in …

We have examined the roles of Asp1018, Glu1027, Arg469 and Asp471 in the allosteric
domain of Rhizobium etli pyruvate carboxylase. Arg469 and Asp471 interact directly with the …