Detection of alternative DNA structures and its implications for human disease

G Matos-Rodrigues, JA Hisey, A Nussenzweig… - Molecular Cell, 2023 - cell.com
Around 3% of the genome consists of simple DNA repeats that are prone to forming
alternative (non-B) DNA structures, such as hairpins, cruciforms, triplexes (H-DNA), four …

Molecular insights into regulatory RNAs in the cellular machinery

S Yang, SH Kim, E Yang, M Kang, JY Joo - Experimental & Molecular …, 2024 - nature.com
It is apparent that various functional units within the cellular machinery are derived from
RNAs. The evolution of sequencing techniques has resulted in significant insights into …

Nano-DMS-MaP allows isoform-specific RNA structure determination

P Bohn, AS Gribling-Burrer, UB Ambi, RP Smyth - Nature Methods, 2023 - nature.com
Genome-wide measurements of RNA structure can be obtained using reagents that react
with unpaired bases, leading to adducts that can be identified by mutational profiling on next …

Interactive visualization of nanopore sequencing signal data with Squigualiser

H Samarakoon, K Liyanage, JM Ferguson… - …, 2024 - academic.oup.com
Motivation Nanopore sequencing current signal data can be 'basecalled'into sequence
information or analysed directly, with the capacity to identify diverse molecular features, such …

Isoform-specific RNA structure determination using Nano-DMS-MaP

AS Gribling-Burrer, P Bohn, RP Smyth - Nature Protocols, 2024 - nature.com
RNA structure determination is essential to understand how RNA carries out its diverse
biological functions. In cells, RNA isoforms are readily expressed with partial variations …

Direct RNA sequencing in plants: practical applications and future perspectives

XT Zhu, P Sanz-Jimenez, XT Ning, MT ul Qamar… - Plant …, 2024 - cell.com
The transcriptome serves as a bridge that links genomic variation and phenotype diversity. A
vast number of studies using next-generation RNA sequencing (RNA-seq) in the last two …

Design approaches to expand the toolkit for building cotranscriptionally encoded RNA strand displacement circuits

SW Schaffter, ME Wintenberg, TM Murphy… - ACS Synthetic …, 2023 - ACS Publications
Cotranscriptionally encoded RNA strand displacement (ctRSD) circuits are an emerging tool
for programmable molecular computation, with potential applications spanning in vitro …

Comprehensive transcriptome sequencing of silkworm Midguts: Uncovering extensive isoform diversity and alternative splicing in BmNPV-Sensitive and BmNPV …

M Tang, Y Liu, H Zhang, L Sun, P Lü, K Chen - Journal of Invertebrate …, 2024 - Elsevier
The silkworm, Bombyx mori, stands out as one of the few economically valuable insects
within the realm of model organisms. However, Bombyx mori nucleopolyhedrovirus …

[HTML][HTML] Probing RNA structure and dynamics using nanopore and next generation sequencing

E Bose, S Xiong, AN Jones - Journal of Biological Chemistry, 2024 - Elsevier
It has become increasingly evident that the structures RNAs adopt are conformationally
dynamic; the various structured states that RNAs sample govern their interactions with other …

[PDF][PDF] Interactive visualization of nanopore sequencing signal data with Squigualiser

S Parameswaran, H Gamaarachchi, IW Deveson - Bioinformatics, 2024 - oxfordjournals.org
Motivation: Nanopore sequencing current signal data can be 'basecalled'into sequence
information or analysed directly, with the capacity to identify diverse molecular features, such …