The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

Genetically engineered T cell immunotherapies have provided remarkable clinical success
to treat B cell acute lymphoblastic leukaemia by harnessing a patient's own T cells to kill …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

Replication fork reversal safeguards genome integrity as a replication stress response. DNA
translocases and the RAD51 recombinase catalyze reversal. However, it remains unknown …

Multiplex assays of variant effect (MAVEs), such as deep mutational scans and massively
parallel reporter assays, test thousands of sequence variants in a single experiment. Despite …

Recombinant adeno-associated viruses (AAV) are under intensive investigation in
numerous clinical trials after they have emerged as a highly promising vector for human …

A lack of tools to precisely control gene expression has limited our ability to evaluate
relationships between expression levels and phenotypes. Here, we describe an approach to …

Chimeric antigen receptor (CAR) T cells have emerged as a promising treatment for patients
with advanced B-cell cancers. However, widespread application of the therapy is currently …

The rise of systems biology has ushered a new paradigm: the view of the cell as a system
that processes environmental inputs to drive phenotypic outputs. Synthetic biology provides …

Reprogramming human fibroblasts to induced pluripotent stem cells (iPSCs) is inefficient,
with heterogeneity among transcription factor (TF) trajectories driving divergent cell states …