To the Editor—The standardization, portability and reproducibility of analysis pipelines are
key issues within the bioinformatics community. Most bioinformatics pipelines are designed …

Transcriptomic changes in specific brain regions can influence the risk of alcohol use
disorder (AUD), but the underlying mechanism is not fully understood. We investigated AUD …

Background The emergence of high throughput technologies that produce vast amounts of
genomic data, such as next-generation sequencing (NGS) is transforming biological …

Genome annotation plays a crucial role in providing comprehensive catalog of genes and
transcripts for a particular species. As research projects generate new transcriptome data …

With the development of single-cell RNA sequencing (scRNA-seq) technology, it has
become possible to perform large-scale transcript profiling for tens of thousands of cells in a …

G Protein Suppressor 2 (GPS2) is a multifunctional protein that exerts important roles in
inflammation and metabolism in adipose, liver, and immune cells. GPS2 has recently been …

Background RNA-seq followed by de novo transcriptome assembly has been a
transformative technique in biological research of non-model organisms, but the …

Background RNA sequencing (RNA-seq) is a common and widespread biological assay,
and an increasing amount of data is generated with it. In practice, there are a large number …

Long-read sequencing has revolutionized genome assembly, yielding highly contiguous,
chromosome-level contigs. However, assemblies from some third generation long read …

Reproducibility is essential to Open Science, as there is limited relevance for finding that
cannot be reproduced by independent research groups, regardless of its validity. It is …