The introduction of thermostable polymerases revolutionized the polymerase chain reaction
(PCR) and biotechnology. However, many GC-rich genes cannot be PCR-amplified with …

Age prediction can provide important information about the contributors of biological
evidence left at crime scenes. DNA methylation has been regarded as the most promising …

Gold nanoparticles (AuNPs) have been reported to facilitate double-stranded DNA
dissociation and improve performance of several PCR systems. Here we investigated …

Because it is difficult to differentiate male and female Columbidae birds (eg, Columba livia)
on the basis of morphology, detection of DNA fragments associated with Chromobox …

GC-rich DNA regions were PCR-amplified with Taq DNA polymerase using either the
canonical set of deoxynucleoside triphosphates or mixtures in which the dCTP had been …

The aim of the study was to examine the effects of frequently used polymerase chain
reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal …

目的: 获取肝癌相关基因(hepatoma associated gene, HTA) 的mRNA 分子全长序列并对其可变
剪接进行分析, 检测其转录本在各肝癌细胞系中的表达, 为进一步研究该基因在肝癌发生 …

Tai Lake, an important biodiversity hotspot of the lower reaches of the Yangtze River in
China, possesses its characteristic fish fauna. Barcoding on native species is important for …

Abstract Prader-Willi (PWS) and Angelman (AS) syndromes are clinically distinct
neurodevelopmental genetic diseases with multiple phenotypic manifestations. They are …

PCR amplification of GC-rich regions often leads to low yield and specificity. Addition of PCR-
enhancing compounds is employed in order to overcome these obstacles. PCR-enhancing …