3′-end processing of eukaryotic mRNA: machinery, regulation, and impact on gene expression
V Boreikaitė, LA Passmore - Annual review of biochemistry, 2023 - annualreviews.org
Formation of the 3′ end of a eukaryotic mRNA is a key step in the production of a mature
transcript. This process is mediated by a number of protein factors that cleave the pre …
transcript. This process is mediated by a number of protein factors that cleave the pre …
[HTML][HTML] Context-specific regulation and function of mRNA alternative polyadenylation
S Mitschka, C Mayr - Nature Reviews Molecular Cell Biology, 2022 - nature.com
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate
mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of …
mRNA isoforms with alternative 3′ untranslated regions (UTRs). The expression of …
Recognition of RNA by the S9. 6 antibody creates pervasive artifacts when imaging RNA: DNA hybrids
The S9. 6 antibody is broadly used to detect RNA: DNA hybrids but has significant affinity for
double-stranded RNA. The impact of this off-target RNA binding activity has not been …
double-stranded RNA. The impact of this off-target RNA binding activity has not been …
[HTML][HTML] Elevated pre-mRNA 3′ end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation
Cleavage and polyadenylation (CPA) is responsible for 3′ end processing of eukaryotic
poly (A)+ RNAs and preludes transcriptional termination. JTE-607, which targets CPSF-73 …
poly (A)+ RNAs and preludes transcriptional termination. JTE-607, which targets CPSF-73 …
[HTML][HTML] Evaluation of fully-functionalized diazirine tags for chemical proteomic applications
The use of photo-affinity reagents for the mapping of noncovalent small molecule–protein
interactions has become widespread. Recently, several 'fully-functionalized'(FF) chemical …
interactions has become widespread. Recently, several 'fully-functionalized'(FF) chemical …
RBBP6 activates the pre-mRNA 3′ end processing machinery in humans
V Boreikaite, TS Elliott, JW Chin… - Genes & …, 2022 - genesdev.cshlp.org
Abstract 3′ end processing of most human mRNAs is carried out by the cleavage and
polyadenylation specificity factor (CPSF; CPF in yeast). Endonucleolytic cleavage of the …
polyadenylation specificity factor (CPSF; CPF in yeast). Endonucleolytic cleavage of the …
[PDF][PDF] Surface antigen-guided CRISPR screens identify regulators of myeloid leukemia differentiation
Lack of cellular differentiation is a hallmark of many human cancers, including acute myeloid
leukemia (AML). Strategies to overcome such a differentiation blockade are an approach for …
leukemia (AML). Strategies to overcome such a differentiation blockade are an approach for …
Recent molecular insights into canonical pre-mRNA 3'-end processing
The majority of eukaryotic messenger RNA precursors (pre-mRNAs) undergo cleavage and
polyadenylation at their 3′ end. This canonical 3′-end processing depends on sequence …
polyadenylation at their 3′ end. This canonical 3′-end processing depends on sequence …
Establishing the catalytic and regulatory mechanism of RNA‐based machineries
Ribonucleoprotein (RNP)‐machineries are comprised of intricate networks of long
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
Alternative polyadenylation: An enigma of transcript length variation in health and disease
NK Mohanan, F Shaji, GR Koshre… - Wiley Interdisciplinary …, 2022 - Wiley Online Library
Alternative polyadenylation (APA) is a molecular mechanism during a pre‐mRNA
processing that involves usage of more than one polyadenylation site (PA‐site) generating …
processing that involves usage of more than one polyadenylation site (PA‐site) generating …