Structural investigation of therapeutic antibodies using hydroxyl radical protein footprinting methods

CY Ralston, JS Sharp - Antibodies, 2022 - mdpi.com
Commercial monoclonal antibodies are growing and important components of modern
therapies against a multitude of human diseases. Well-known high-resolution structural …

Flash oxidation (FOX) system: a novel laser-free fast photochemical oxidation protein footprinting platform

JS Sharp, EE Chea, SK Misra, R Orlando… - Journal of the …, 2021 - ACS Publications
Hydroxyl radical protein footprinting (HRPF) is a powerful and flexible technique for probing
changes in protein topography. With the development of the fast photochemical oxidation of …

Structural Analysis of phosphorylation proteoforms in a dynamic heterogeneous system using flash oxidation coupled in-line with ion exchange chromatography

Z Cheng, SK Misra, A Shami, JS Sharp - Analytical chemistry, 2022 - ACS Publications
Protein posttranslational modifications (PTMs) are key modulators of protein structure and
function that often change in a dynamic fashion in response to cellular stimuli. Dynamic …

Dimethylthiourea as a Quencher in Hydroxyl Radical Protein Footprinting Experiments

AA Shami, SK Misra, LM Jones… - Journal of the American …, 2023 - ACS Publications
Hydroxyl radical protein footprinting (HRPF) is a mass-spectrometry-based method for
studying protein structures, interactions, conformations, and folding. This method is based …

Laser-free hydroxyl radical protein footprinting to perform higher order structural analysis of proteins

SR Weinberger, EE Chea, JS Sharp… - JoVE (Journal of Visualized …, 2021 - jove.com
Hydroxyl Radical Protein Footprinting (HRPF) is an emerging and promising higher order
structural analysis technique that provides information on changes in protein structure …

[PDF][PDF] Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 …

OV Ketsa, MO Zelinska, MM Marchenko - Ukr Biochem J, 2022 - ukrbiochemjournal.org
The effect of laser irradiation of rats combined with omega-3 polyunsaturated fatty acids (ω-3
PUFA) administration on the proteins oxidative modification and superoxide dismutase and …

Mass Spectrometry-Based Approaches for the Analysis of Protein Higher Order Structure

XR Liu, Y Huang - JoVE (Journal of Visualized Experiments), 2023 - jove.com
ARTICLES DISCUSSED: Espino, JA, Jones, LM In vivo hydroxyl radical protein footprinting
for the study of protein interactions in Caenorhabditis elegans. Journal of Visualized …

Development of Hydroxyl Radical Protein Footprinting for Challenging Systems

Z Cheng - 2022 - search.proquest.com
Structural biology plays an important role in modern molecular biosciences. High-resolution
hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry (MS) based method …

[PDF][PDF] Research News. Publications, 2020. Volume 4

S Majumdar - 2020 - egrove.olemiss.edu
23. Eissa IH, Ibrahim MK, Metwaly AM, Belal A, Mehany ABM, Abdelhady AA, Elhendawy
MA, Radwan MM, ElSohly MA, Mahdy HA. Design, molecular docking, in vitro, and in vivo …

[引用][C] Oxidative modification of proteins and activity of antioxidant enzymes in the mitochondrial fraction of rat liver under the action of laser irradiation and ω-3 …

OV Ketsa, MO Zelinska, MM Marchenko - 2022