[HTML][HTML] Bacteriophages of Shiga Toxin-Producing Escherichia coli and Their Contribution to Pathogenicity

L Rodríguez-Rubio, N Haarmann, M Schwidder… - Pathogens, 2021 - mdpi.com
Shiga toxins (Stx) of Shiga toxin-producing Escherichia coli (STEC) are generally encoded
in the genome of lambdoid bacteriophages, which spend the most time of their life cycle …

Current and promising approaches to identify horizontal gene transfer events in metagenomes

GM Douglas, MGI Langille - Genome biology and evolution, 2019 - academic.oup.com
High-throughput shotgun metagenomics sequencing has enabled the profiling of myriad
natural communities. These data are commonly used to identify gene families and pathways …

Reekeekee- and roodoodooviruses, two different Microviridae clades constituted by the smallest DNA phages

E Olo Ndela, S Roux, C Henke, A Sczyrba… - Virus …, 2023 - academic.oup.com
Small circular single-stranded DNA viruses of the Microviridae family are both prevalent and
diverse in all ecosystems. They usually harbor a genome between 4.3 and 6.3 kb, with a …

RiboReport-benchmarking tools for ribosome profiling-based identification of open reading frames in bacteria

R Gelhausen, T Müller, SL Svensson… - Briefings in …, 2022 - academic.oup.com
Small proteins encoded by short open reading frames (ORFs) with 50 codons or fewer are
emerging as an important class of cellular macromolecules in diverse organisms. However …

[HTML][HTML] Are antisense proteins in prokaryotes functional?

Z Ardern, K Neuhaus, S Scherer - Frontiers in molecular biosciences, 2020 - frontiersin.org
Many prokaryotic RNAs are transcribed from loci outside of annotated protein coding genes.
Across bacterial species hundreds of short open reading frames antisense to annotated …

[HTML][HTML] Optimality in the standard genetic code is robust with respect to comparison code sets

S Wichmann, Z Ardern - Biosystems, 2019 - Elsevier
The genetic code and its evolution have been studied by many different approaches. One
approach is to compare the properties of the standard genetic code (SGC) to theoretical …

OLGenie: estimating natural selection to predict functional overlapping genes

CW Nelson, Z Ardern, X Wei - Molecular Biology and Evolution, 2020 - academic.oup.com
Purifying (negative) natural selection is a hallmark of functional biological sequences, and
can be detected in protein-coding genes using the ratio of nonsynonymous to synonymous …

[HTML][HTML] Non-canonical transcriptional start sites in E. coli O157:H7 EDL933 are regulated and appear in surprisingly high numbers

B Zehentner, S Scherer, K Neuhaus - BMC microbiology, 2023 - Springer
Abstract Analysis of genome wide transcription start sites (TSSs) revealed an unexpected
complexity since not only canonical TSS of annotated genes are recognized by RNA …

[HTML][HTML] Recommendations for bacterial ribosome profiling experiments based on bioinformatic evaluation of published data

A Glaub, C Huptas, K Neuhaus, Z Ardern - Journal of Biological Chemistry, 2020 - ASBMB
Ribosome profiling (RIBO-Seq) has improved our understanding of bacterial translation,
including finding many unannotated genes. However, protocols for RIBO-Seq and …

Hundreds of Out-of-Frame Remodeled Gene Families in the Escherichia coli Pangenome

AK Watson, P Lopez, E Bapteste - Molecular Biology and …, 2022 - academic.oup.com
All genomes include gene families with very limited taxonomic distributions that potentially
represent new genes and innovations in protein-coding sequence, raising questions on the …