Biomedicine is one of the fastest growing areas of additive manufacturing. Especially, in the
field of in vitro diagnostics (IVD), contributions of 3D printing include i) rapid prototyping and …

Digital nucleic acid amplification technologies (dNAATs) have become an emerging platform
for pathogen detection. It is not only suitable for the absolute quantitative analysis of target …

Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most
existing platforms are dependent on large auxiliary equipment (eg, vacuum pump …

Cell-free DNA (cfDNA) methylation profiling is considered promising and potentially reliable
for liquid biopsy to study progress of diseases and develop reliable and consistent …

Epigenetic transformations may provide early indicators for cancer and other disease.
Specifically, the amount of genomic 5‐hydroxymethylcytosine (5‐hmC) was shown to be …

Background Tuberculosis is still a significant diagnostic and therapeutic challenge with high
proportion of smear-and culture-negative incidences worldwide. The conventional …

DNA methylation is a common epigenetic modification, and the current commonly used
methods for DNA methylation detection include methylation-specific PCR, methylation …

The diagnosis of pleural tuberculosis (TB) remains difficult due to the paucity of
Mycobacterium tuberculosis in pleural fluid (PF). This study aimed to improve pleural TB …

Objectives Tuberculous meningitis (TBM) is difficult to diagnose. Digital PCR (dPCR) is a
novel method which can quantify trace nucleic acids. This study sought to evaluate the …

Background Promoter hypermethylation is one of the enabling mechanisms of hallmarks of
cancer. Tumor suppressor genes like RARB and GSTP1 have been reported as …