Capillary electrophoresis: trends and recent advances

RLC Voeten, IK Ventouri, R Haselberg… - Analytical …, 2018 - ACS Publications
Since the introduction of modern capillary electrophoresis (CE) by Jorgenson and Lukacs in
1981, CE has evolved into a highly mature and versatile separation technique. After a first …

How many human proteoforms are there?

R Aebersold, JN Agar, IJ Amster, MS Baker… - Nature chemical …, 2018 - nature.com
Despite decades of accumulated knowledge about proteins and their post-translational
modifications (PTMs), numerous questions remain regarding their molecular composition …

Top-down proteomics: ready for prime time?

B Chen, KA Brown, Z Lin, Y Ge - Analytical chemistry, 2017 - ACS Publications
Beyond the genetic code, the human proteome is greatly diversified by genetic variations,
alternative splicing of RNA transcripts, and post-translational modifications (PTMs). 6, 7 In …

Capillary electrophoresis separations of glycans

G Lu, CL Crihfield, S Gattu, LM Veltri… - Chemical …, 2018 - ACS Publications
Capillary electrophoresis has emerged as a powerful approach for carbohydrate analyses
since 2014. The method provides high resolution capable of separating carbohydrates by …

The diverse and expanding role of mass spectrometry in structural and molecular biology

P Lössl, M van de Waterbeemd, AJR Heck - The EMBO journal, 2016 - embopress.org
The emergence of proteomics has led to major technological advances in mass
spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput …

[HTML][HTML] Capillary electrophoresis and the biopharmaceutical industry: Therapeutic protein analysis and characterization

H Kaur, J Beckman, Y Zhang, ZJ Li, M Szigeti… - TrAC Trends in …, 2021 - Elsevier
Capillary electrophoresis (CE) has emerged as a powerful technique for comprehensive
physicochemical characterization of monoclonal antibodies (mAbs) as well as other …

[HTML][HTML] Advances in capillary electrophoresis for the life sciences

JS Toraño, R Ramautar, G de Jong - Journal of Chromatography B, 2019 - Elsevier
Capillary electrophoresis (CE) played an important role in developments in the life sciences.
The technique is nowadays used for the analysis of both large and small molecules in …

Applications of Raman spectroscopy in biopharmaceutical manufacturing: a short review

K Buckley, AG Ryder - Applied spectroscopy, 2017 - journals.sagepub.com
The production of active pharmaceutical ingredients (APIs) is currently undergoing its
biggest transformation in a century. The changes are based on the rapid and dramatic …

Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research

FP Gomes, JR Yates III - Mass spectrometry reviews, 2019 - Wiley Online Library
Progress in proteomics research has led to a demand for powerful analytical tools with high
separation efficiency and sensitivity for confident identification and quantification of proteins …

MS‐based glycomics and glycoproteomics methods enabling isomeric characterization

W Peng, CD Gutierrez Reyes, S Gautam… - Mass spectrometry …, 2023 - Wiley Online Library
Glycosylation is one of the most significant and abundant posttranslational modifications in
mammalian cells. It mediates a wide range of biofunctions, including cell adhesion, cell …