Understanding the mechanisms by which chromatin structure controls eukaryotic
transcription has been an intense area of investigation for the past 25 years. Many of the key …

Regulation of gene expression in eukaryotes is an extremely complex process. In this
review, we break down several critical steps, emphasizing new data and techniques that …

Mechanical deformations of DNA such as bending are ubiquitous and have been implicated
in diverse cellular functions. However, the lack of high-throughput tools to measure the …

Genome editing with CRISPR-Cas nucleases has been applied successfully to a wide range
of cells and organisms. There is, however, considerable variation in the efficiency of …

Spatiotemporal patterns of gene expression are fundamental to every developmental
program. The resulting macroscopic domains have been mainly characterized by their levels …

We have combined standard micrococcal nuclease (MNase) digestion of nuclei with a
modified protocol for constructing paired-end DNA sequencing libraries to map both …

Nitric oxide (NO) is a signaling molecule involved in plant response to various abiotic
stresses. However, the application of NO donors in agriculture is hampered by the instability …

Fifty per cent of the genome is discontinuously replicated on the lagging strand as Okazaki
fragments. Eukaryotic Okazaki fragments remain poorly characterized and, because …

Nucleosomes present a barrier for the binding of most transcription factors (TFs). However,
special TFs known as nucleosome-displacing factors (NDFs) can access embedded sites …

Immunity relies on the heterogeneity of immune cells and their ability to respond to pathogen
challenges. In the adaptive immune system, lymphocytes display a highly diverse antigen …