Enumerating soil biodiversity

MA Anthony, SF Bender… - Proceedings of the …, 2023 - pnas.org
Soil is an immense habitat for diverse organisms across the tree of life, but just how many
organisms live in soil is surprisingly unknown. Previous efforts to enumerate soil biodiversity …

Metabolic exchanges are ubiquitous in natural microbial communities

C Kost, KR Patil, J Friedman, SL Garcia… - Nature Microbiology, 2023 - nature.com
Microbial communities drive global biogeochemical cycles and shape the health of plants
and animals—including humans. Their structure and function are determined by ecological …

[HTML][HTML] Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation

S Liu, CD Moon, N Zheng, S Huws, S Zhao, J Wang - Microbiome, 2022 - Springer
Although there is now an extensive understanding of the diversity of microbial life on earth
through culture-independent metagenomic DNA sequence analyses, the isolation and …

A global atlas of the dominant bacteria found in soil

M Delgado-Baquerizo, AM Oliverio, TE Brewer… - Science, 2018 - science.org
The immense diversity of soil bacterial communities has stymied efforts to characterize
individual taxa and document their global distributions. We analyzed soils from 237 …

High proportions of bacteria and archaea across most biomes remain uncultured

AD Steen, A Crits-Christoph, P Carini… - The ISME …, 2019 - academic.oup.com
A recent paper by Martiny argues that “high proportions” of bacteria in diverse Earth
environments have been cultured. Here we reanalyze a portion of the data in that paper, and …

Microplastics from mulching film is a distinct habitat for bacteria in farmland soil

M Zhang, Y Zhao, X Qin, W Jia, L Chai, M Huang… - Science of the Total …, 2019 - Elsevier
Microplastics, as an emerging pollutant of global importance, have been well documented in
aquatic ecosystems. However, little is known about the effects of microplastics on …

Uncultivated microbes in need of their own taxonomy

KT Konstantinidis, R Rosselló-Móra… - The ISME journal, 2017 - academic.oup.com
The great majority of microbial species remains uncultured, severely limiting their taxonomic
characterization and thus communication among scientists. Although Candidatus was …

[HTML][HTML] Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system

K Anantharaman, CT Brown, LA Hug, I Sharon… - Nature …, 2016 - nature.com
The subterranean world hosts up to one-fifth of all biomass, including microbial communities
that drive transformations central to Earth's biogeochemical cycles. However, little is known …

[HTML][HTML] GUNC: detection of chimerism and contamination in prokaryotic genomes

A Orakov, A Fullam, LP Coelho, S Khedkar… - Genome biology, 2021 - Springer
Genomes are critical units in microbiology, yet ascertaining quality in prokaryotic genome
assemblies remains a formidable challenge. We present GUNC (the Genome UNClutterer) …

OptiClust, an improved method for assigning amplicon-based sequence data to operational taxonomic units

SL Westcott, PD Schloss - MSphere, 2017 - Am Soc Microbiol
Assignment of 16S rRNA gene sequences to operational taxonomic units (OTUs) is a
computational bottleneck in the process of analyzing microbial communities. Although this …