Isothermal amplification of nucleic acids: The race for the next “gold standard”
BB Oliveira, B Veigas, PV Baptista - Frontiers in Sensors, 2021 - frontiersin.org
Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …
Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review
P Craw, W Balachandran - Lab on a Chip, 2012 - pubs.rsc.org
Nucleic Acid Testing (NAT) promises rapid, sensitive and specific diagnosis of infectious,
inherited and genetic disease. The next generation of diagnostic devices will interrogate the …
inherited and genetic disease. The next generation of diagnostic devices will interrogate the …
Isothermal nucleic acid amplification techniques and their use in bioanalysis
OL Bodulev, IY Sakharov - Biochemistry (Moscow), 2020 - Springer
Recently, there has been a rapid progress in the development of techniques for isothermal
amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The …
amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The …
Isothermal amplification technology for disease diagnosis
P Boonbanjong, K Treerattrakoon, W Waiwinya… - Biosensors, 2022 - mdpi.com
Isothermal amplification (IA) is a nucleic acid amplification technology (NAAT) that has
contributed significantly to the healthcare system. The combination of NAAT with a suitable …
contributed significantly to the healthcare system. The combination of NAAT with a suitable …
Diagnostic devices for isothermal nucleic acid amplification
Since the development of the polymerase chain reaction (PCR) technique, genomic
information has been retrievable from lesser amounts of DNA than previously possible. PCR …
information has been retrievable from lesser amounts of DNA than previously possible. PCR …
Rolling circle amplification as isothermal gene amplification in molecular diagnostics
NI Goo, DE Kim - Biochip journal, 2016 - Springer
Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an
efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic …
efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic …
Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation
Introduction Suitable sample collection and preparation methods are essential to enable
nucleic acid amplification testing at the point of care (POC). Strategies that allow direct …
nucleic acid amplification testing at the point of care (POC). Strategies that allow direct …
Integration of microbead DNA handling with optomagnetic detection in rolling circle amplification assays
Rolling circle amplification (RCA) is a linear isothermal amplification technique that is widely
applied in biomolecular assays due to its high specificity. Handling of a target sample using …
applied in biomolecular assays due to its high specificity. Handling of a target sample using …
Recent developments in nucleic acid identification using solid-phase enzymatic assays
DA Khodakov, AV Ellis - Microchimica Acta, 2014 - Springer
Abstract This review (containing 101 refs.) covers recent achievements in the development
of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We …
of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We …
Rolling circle amplification-based biosensors
L Ou, A Sun, K Liu - Analytical Letters, 2015 - Taylor & Francis
Rolling circle amplification is an isothermal method for amplifying DNA. The technique
rapidly synthesizes a long (more than 1000 bases), linear, tandemly-repetitive single …
rapidly synthesizes a long (more than 1000 bases), linear, tandemly-repetitive single …