In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …

Biological macromolecules function in highly crowded cellular environments. The structure
and dynamics of proteins and nucleic acids are well characterized in vitro, but in vivo …

Interactions between proteins are orchestrated in a precise and time-dependent manner,
underlying cellular function. The binding affinity, defined as the strength of these …

Analysis of the human genome reveals that approximately a third of all open reading frames
code for proteins that enter the endoplasmic reticulum (ER), demonstrating the importance of …

Protein structural changes induced by external perturbations or internal cues can profoundly
influence protein activity and thus modulate cellular physiology. A number of biophysical …

Abstract Changes in protein conformation can affect protein function, but methods to probe
these structural changes on a global scale in cells have been lacking. To enable large-scale …