The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Bioconjugation methodologies have proven to play a central enabling role in the recent
development of biotherapeutics and chemical biology approaches. Recent endeavours in …

A historical overview of peptide chemistry from T. Curtius to E. Fischer to M. Bergmann and
L. Zervas is first presented. Next, the fundamentals of peptide synthesis with a focus on solid …

As a versatile therapeutic modality, peptides attract much attention because of their great
binding affinity, low toxicity, and the capability of targeting traditionally “undruggable” protein …

The broad utility of native chemical ligation (NCL) in protein synthesis has fostered a search
for methods that enable the efficient synthesis of C-terminal peptide-thioesters, key …

Vibrational spectroscopy provides a direct route to the physicochemical characterization of
molecules. While both IR and Raman spectroscopy have been used for decades to provide …

Highlights•Recent progress in ligation chemistry for the synthesis of peptides and proteins is
outlined.•Extensions of native chemical ligation at thiol and selenol amino acids.•New C …

Native chemical ligation followed by desulfurization is a powerful strategy for the assembly
of proteins. Here we describe the development of a high-yielding, one-pot ligation …

The article presents a study of chemoselective peptide ligation-desulfurization to a protected
β-mercapto aspartate (Asp) residue. It develops an scalable route to a protected β-mercapto …

Native chemical ligation enables the chemical synthesis of proteins. Previously, thiol‐
containing auxiliary groups have been used to extend the reaction scope beyond N‐terminal …